Identification of glucosinolate QTLs in Brassica oleracea C genome revealed interesting genome regions for biological applications
Reem Issa
Applied Science University, College of Pharmacy, Amman, Jordan
Abstract:
Glucosinolates are a group of secondary plant metabolites, which have been shown to play important roles in human health and nutrition. The Brassicaceae family consists of vegetable crops of biological, economical and agricultural importance attributed to glucosinolates where their activity is largely determined by their content. Identification of novel genes or regulators of expression are important for optimising the glucosinolate composition of Brassica crops. We developed an optimised HPLC-UV/ESI-MS/MS methodology for identification and quantification of desulfated glucosinolates within leaf material. In all plant lines, greater variation in the concentration was observed within the aliphatic glucosinolates than within indolic glucosinolates. A comparison of the relative glucosinolate content determined from the plant lines in our study with kale, broccoli and cauliflower values in the literature revealed interesting variations in the glucosinolate profiles synthesized by these vegetable crops, revealed some interesting plant lines that have potential biological applications. This methodology was then used to identify Quantitative Trait Loci for individual glucosinolates and for key points in their biosynthesis. A major gene effect was found near the top of B. oleracea LG9 associated with aliphatic glucosinolate synthesis. Consequently, this information can be applied to other Brassica species for breeding vegetable crops with modified glucosinolate profiles.
